Target
Human RPA70 / RPA1
Synonyms
RPA1 | HSSB | MST075 | Replication factor A protein 1 | Replication protein A1 (70kD) | RF-A | RF-A protein 1 | MSTP075 | Replication protein A1, 70kDa | RP-A | REPA1 | RP-A p70 | RPA70
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CKSYEDATKITVRSN, from the internal region of the protein sequence according to NP_002936.1.
Specificity
Human RPA70 / RPA1
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70+80kD band observed in lysates of cell line HeLa (calculated MW of 68.1kD according to NP_002936.1). The upper band likely represents the acetylated protein. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee