Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone G28-8] (IgG1) to Human RP105 / CD180
Mouse Monoclonal [clone MHR73] (IgG1) to Human RP105 / CD180
IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry, Functional Assay (applications tested for the base form of this product only)
Human RP105 / CD180
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MHR73]
Also available conjugated with FITC.
IHC - Paraffin
IHC - Frozen
(applications tested for the base form of this product only)
Specificity and Use
Human CD180 / RP105
For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity dilutions have to be made according to the amounts RP150 to be inactivated.
CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria.