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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RP105 / CD180 Antibody (clone G28‑8, PE) LS‑C204446
CD180 antibody LS-C204446 is a PE-conjugated mouse monoclonal antibody to human CD180 (RP105). Validated for Flow, Func and IP.
Catalog
Size
Price
LS-C204446-100
100 tst
$315
Description
CD180 antibody LS-C204446 is a PE-conjugated mouse monoclonal antibody to human CD180 (RP105). Validated for Flow, Func and IP.
Target
Human RP105 / CD180
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [G28-8]
Conjugations
Phycoerythrin. Also available Unconjugated or conjugated with APC.
Modifications
Unmodified
Applications
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
  • (applications tested for the base form of this product only)
Immunogen
RP105 / CD180 antibody was raised against human tonsillar B cells
Specificity
The mouse monoclonal antibody G28-8 recognizes CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
Usage
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 15 mM Sodium Azide
Storage
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About RP105 / CD180
Q99467 NM_005582 NP_005573.2

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Surface staining of normal human peripheral blood cells with anti-human CD180 (MHR73-11) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.
Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.
Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.
Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.

Flow Cytometry

Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.
Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.

Requested From: United States
Date Requested: 12/15/2018
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