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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ROBO4 Antibody (C‑Terminus, Biotin) LS‑C141593
ROBO4 antibody LS-C141593 is a biotin-conjugated rabbit polyclonal antibody to human ROBO4. Validated for IHC and WB.
Catalog
Size
Price
LS-C141593-100
100 µl
Unavailable

Popular ROBO4 Products

Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6693 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Roundabout 4 Antibody IHC of formalin-fixed and paraffin-embedded prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody for immunohistochemistry.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA

Product Description

ROBO4 antibody LS-C141593 is a biotin-conjugated rabbit polyclonal antibody to human ROBO4. Validated for IHC and WB.
About ROBO4
Roundabout (Robo) is a class of neural guidance receptors that bind slit ligands and mediated axonal repulsion and inhibition of leukocyte migrations. In addition to neural expression, Robo4 shows expression in endothelial cells of the vasculature, placenta, and tumors. It has been shown to play a role in embryonic angiogenesis. Studies in mice showed expression restricted to endothelial cells. Q8WZ75 NM_019055 NP_061928.4

ROBO4 Antibody, ECSM4 Antibody, Magic roundabout Antibody, Roundabout homolog 4 Antibody, MRB Antibody


Specifications

Target
Human ROBO4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ROBO4 antibody was raised against a synthetic peptide made to a C-terminal region of the human protein (within residues 900-1007). [Swiss-Prot# Q8WZ75]
Epitope
C-Terminus
Specificity
This recognizes the 85 kD and 107 kD isoforms of the ROBO4 human protein.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.05% sodium azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of ROBO4 in HUVEC cell lysates with LS-B6694.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of ROBO4 in HUVEC cell lysates with LS-B6694. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of ROBO4 in HUVEC cell lysates with LS-B6694.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of ROBO4 in HUVEC cell lysates with LS-B6694. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of ROBO4 in HUVEC cell lysates with LS-B6694.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of ROBO4 in HUVEC cell lysates with LS-B6694. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ROBO4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6694 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of ROBO4 in HUVEC cell lysates with LS-B6694.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of ROBO4 in HUVEC cell lysates with LS-B6694. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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