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Anti-RNH1 Antibody (clone AT1H23) IHC-plus™ LS-B3585

Note: This antibody replaces LS-C103490

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNH1 Antibodies

Anti-RNH1 Antibody (clone AT1H23) IHC-plus™ LS-B3585
Mouse Monoclonal [clone AT1H23] (IgG2a,k) to Human RNH1
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RNH1 Antibody (aa251-300) IHC-plus™ LS-B11534
Rabbit Polyclonal (IgG) to Human RNH1
Human, Monkey, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RNH1 Antibody (aa151-200) IHC-plus™ LS-B11565
Rabbit Polyclonal to Human RNH1
Human, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AT1H23] (IgG2a,k) to Human RNH1
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human RNH1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [AT1H23]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

RNH1 antibody was raised against recombinant Ribonuclease inhibitor 1(RNH1) (7-461aa) purified from E. coli
Ribonuclease inhibitor 1(RNH1) (7-461aa)
Immunohistochemistry: LS-B3585 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3585 was determined to be 5 ug/ml. Western Blot: Extracts of mouse liver (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About RNH1

P13489 NM_002939 NP_002930.2

RNH1 Antibody, Placental RNase inhibitor Antibody, PRI Antibody, RAI Antibody, Ribonuclease inhibitor Antibody, RNH Antibody

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form.

Immunohistochemistry

Immunohistochemistry
Anti-RNH1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3585 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-RNH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3585 concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/10/2016

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