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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RNF8 Antibody (aa2‑14) LS‑C55414
RNF8 antibody LS-C55414 is an unconjugated goat polyclonal antibody to human RNF8. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55414-100
100 µg
Unavailable

Popular RNF8 Products

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Immunohistochemistry of paraffin-embedded human testicle using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

RNF8 antibody LS-C55414 is an unconjugated goat polyclonal antibody to human RNF8. Validated for Peptide-ELISA and WB.
About RNF8
E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. O76064 NM_003958 NP_003949.1

RNF8 Antibody, C3HC4-type zinc finger protein Antibody, KIAA0646 Antibody, HRNF8 Antibody, RING finger protein 8 Antibody


Specifications

Target
Human RNF8
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:64000) & (0.1 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
RNF8 antibody was raised against synthetic peptide GEPGFFVTGDRAG-C from the N-terminus of human RNF8 (NP_003949.1; NP_898901.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan (100%); Gibbon, Monkey (92%).
Blocking Peptide
LS-E25497 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55414. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-14
Specificity
Human RNF8. This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Customer Reviews (0)


Images


Western blot

Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (0.1?g/ml) of Human Lung lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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