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Anti-RNF180 Antibody (Internal) LS-C155600

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNF180 Antibodies

Anti-RNF180 Antibody (aa401-430, AP) LS-C268389
Rabbit Polyclonal (IgG) to Human RNF180
Human
IHC, Western blot, ELISA
AP Conjugated
Anti-RNF180 Antibody (aa401-430, APC) LS-C268390
Rabbit Polyclonal (IgG) to Human RNF180
Human
IHC, Western blot, ELISA
APC Conjugated
Anti-RNF180 Antibody (aa401-430, Biotin) LS-C268392
Rabbit Polyclonal (IgG) to Human RNF180
Human
IHC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RNF180
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human RNF180
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

RNF180 antibody was raised against recombinant fragment corresponding to a region within amino acids 120 and 438 of RNF180 (SwissProt Q86T96). Percent identity by BLAST analysis: Human (100%); Rat (80%).
Internal
Human RNF180
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
For research use only.

About RNF180

Q86T96 Q86T96

RNF180 Antibody, Ring finger protein 180 Antibody

E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded HBL435 xenograft, using RNF180 antibody at 1:500 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE RNF180 antibody diluted at 1:2000

Requested From: United States
Date Requested: 12/4/2016

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