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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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RNF138 Antibody (C‑Terminus) LS‑C80038 Best Seller
RNF138 antibody LS-C80038 is an unconjugated rabbit polyclonal antibody to RNF138 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80038-50
50 µg
Unavailable

Popular RNF138 Products

RNF138 Antibody western blot of T47D cell line lysates (35 ug/lane). The RNF138 antibody detected the RNF138 protein (arrow).
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Product Description

RNF138 antibody LS-C80038 is an unconjugated rabbit polyclonal antibody to RNF138 from human, mouse, rat and other species. Validated for WB.
About RNF138
E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway. Q8WVD3 NM_016271 NP_057355.2

RNF138 Antibody, HNARF Antibody, HSD-4 Antibody, RING finger protein 138 Antibody, STRIN Antibody


Specifications

Target
Human RNF138
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
RNF138 antibody was raised against synthetic peptide from C-Terminus of human RNF138 (Q8WVD3, NP_057355). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%); Turkey, Zebra finch, Chicken (92%); Hamster (84%).
Blocking Peptide
LS-E11625 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80038. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human RNF138
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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