Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RNLQSRWRSPSHEN, from the C-Terminus of protein sequence according to NP_060621.3NP_001171134.1.
Specificity
Human RIF1. This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1
Applications
- Immunofluorescence (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 270kD band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kD according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee