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Anti-RIC8A Antibody (clone 3G3) LS-C115097

Ordering

Wt. Vol. Conc. Price
- 100 µl - Unavailable

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3G3] (IgG1) to Human RIC8A
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human RIC8A
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3G3]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)

Specificity and Use

RIC8A antibody was raised against full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.
Human RIC8A
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.05% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RIC8A

Q9NPQ8 NM_021932 NP_068751.4

RIC8A Antibody, Protein Ric-8A Antibody, Synembryn Antibody, Synembryn-A Antibody, RIC8 Antibody

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex By similarity.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Kidney tissue using anti-RIC8A mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescence
Anti-RIC8A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of HepG2 cells using anti-RIC8A mouse monoclonal antibody.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8A.

Requested From: 
Date Requested: 3/30/2017

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