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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RHOA Antibody (clone 1B12) IHC‑plus™ LS‑B6004 Best Seller
Note: This antibody replaces LS-C133050
RHOA antibody LS-B6004 is an unconjugated mouse monoclonal antibody to human RHOA. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6004-50
50 µg (0.5 mg/ml)
Unavailable

Popular RHOA Products

Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6005 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Western blot analysis of extracts of various cell lines.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
IHC (P) using RhoA + RhoC Antibody (1B3-4A10)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: ICC, Immunofluorescence, Flow Cytometry, ELISA

Product Description

RHOA antibody LS-B6004 is an unconjugated mouse monoclonal antibody to human RHOA. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About RHOA
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. P61586 NM_001664 NP_001655.1

RHOA Antibody, ARH12 Antibody, ARHA Antibody, Aplysia ras-related homolog 12 Antibody, H12 Antibody, Oncogene RHO H12 Antibody, Rho cDNA clone 12 Antibody, RHO12 Antibody, RHOH12 Antibody, Ras homolog family member A Antibody, Small GTP binding protein RhoA Antibody, Transforming protein RhoA Antibody


Specifications

Target
Human RHOA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,l Monoclonal [1B12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RHOA antibody was raised against rHOA (AAH01360, 1 a.a. ~ 194 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human RHOA
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunohistochemistry

Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of RHOA expression in HL-60 cell lysate.
Western blot of RHOA expression in HL-60 cell lysate.

Western blot

Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunohistochemistry

Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of RHOA expression in HL-60 cell lysate.
Western blot of RHOA expression in HL-60 cell lysate.

Western blot

Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunohistochemistry

Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of RHOA expression in HL-60 cell lysate.
Western blot of RHOA expression in HL-60 cell lysate.

Western blot

Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunohistochemistry

Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.
Anti-RHOA antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6004 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of RHOA expression in HL-60 cell lysate.
Western blot of RHOA expression in HL-60 cell lysate.

Western blot

Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.
Western blot of RHOA expression in transfected 293T cell line. Lane 1: RHOA transfected lysate(21.8 KDa). Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RHOA is approximately 10 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/25/2018

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