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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RGS1 Antibody (aa198‑209) IHC‑plus™ LS‑B3594
Note: This antibody replaces LS-C55386
RGS1 antibody LS-B3594 is an unconjugated goat polyclonal antibody to RGS1 from human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3594-50
50 µg (0.5 mg/ml)
Unavailable

Popular RGS1 Products

Anti-RGS1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1984 concentration 20 ug/ml.
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Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7701 dilution 10 ug/ml.
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Applications: IHC, Western blot, ELISA

Product Description

RGS1 antibody LS-B3594 is an unconjugated goat polyclonal antibody to RGS1 from human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About RGS1
RGS1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. Q08116 NM_002922 NP_002913.3

RGS1 Antibody, 1R20 Antibody, B-cell activation protein BL34 Antibody, BL34 Antibody, Early response protein 1R20 Antibody, IER1 Antibody, IR20 Antibody


Specifications

Target
Human RGS1
Host
Goat
Reactivity
Human, Monkey, Bat, Bovine, Horse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RGS1 antibody was raised against synthetic peptide C-NLLNDLQANSLK from the C-terminus of human RGS1 (NP_002913.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bovine, Bat, Horse (100%); Mouse, Rat, Panda, Dog, Rabbit, Platypus (92%).
Blocking Peptide
LS-E25348 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3594. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa198-209
Specificity
Human RGS1.
Usage
Immunohistochemistry: LS-B3594 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3594 was determined to be 2.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Western blot

Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Western blot

Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Western blot

Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.
Anti-RGS1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3594 concentration 2.5 ug/ml.

Western blot

Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (1?g/ml) of HepG2 lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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