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Anti-RFP / Red Fluorescent Protein Antibody (FITC) LS-C154267

Catalog Size Price
LS-C154267-100 100 µg Unavailable

Most Popular RFP / Red Fluorescent Protein Antibodies

Anti-RFP / Red Fluorescent Protein Antibody LS-C17622
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Anti-RFP Antibody - Western Blot. Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kD band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2500 for 1 h at RT followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Immunocytochemistry Image

100% Guaranteed
Rabbit Polyclonal (IgG) to Discosoma RFP / Red Fluorescent Protein
Immunofluorescence, Western blot, ELISA
FITC Conjugated


Discosoma RFP / Red Fluorescent Protein
Discosoma (tested or 100% immunogen sequence identity)
IgG Polyclonal
Affinity purified


  • Immunofluorescence (1:500 - 1:2500)
  • Western blot (1:10000)
  • ELISA (1:20000)

Specificity and Use

The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.

About RFP / Red Fluorescent Protein

Red fluorescent protein (RFP) was originally isolated from Discosoma coral as a tetramer referred to as dsRed. Several synthetic variants of the protein have been created, including monomers. It is commonly used to examine intercellular processes such as tracking gene expression and tagging other proteins.

Requested From: 
Date Requested: 5/23/2018

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