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Anti-RFC5 Antibody (aa170-200) LS-C97299

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RFC5 Antibodies

Anti-RFC5 Antibody (N-Terminus) LS-C80848
Rabbit Polyclonal (IgG) to Human RFC5
Human, Monkey, Dog, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RFC5 Antibody LS-C90983
Goat Polyclonal to Human RFC5
Human
Western blot, ELISA
Unconjugated
Anti-RFC5 Antibody LS-C409595
Rabbit Polyclonal (IgG) to Human RFC5
Human
IHC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RFC5
Human
Western blot, Flow Cytometry
Unconjugated

Details

Human RFC5
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

RFC5 antibody was raised against kLH-conjugated synthetic peptide between amino acids 170-200 from internal region of human RFC5.
LS-E7120 - Lyophilized - 100 µg - $145.00
aa170-200

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About RFC5

P40937 NM_007370 NP_031396.1

RFC5 Antibody, Activator 1 subunit 5 Antibody, A1 36 kDa subunit Antibody, Activator 1 36 kDa subunit Antibody, RF-C 36 kDa subunit Antibody, RFC, 36.5 kD subunit Antibody, RFC36 Antibody, Replication factor C subunit 5 Antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits.

Western blot

Western blot
Western blot of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.

Western blot

Western blot
Western blot of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35 ug/lane). RFC5(arrow) was detected using the purified antibody( Provided by Hirotaka Ata).

Western blot

Western blot
Western blot of anti-RFC5 Antibody (Center R185) in HeLa cell line lysates (35 ug/lane). RFC5(arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
RFC5 Antibody (Center R185) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/6/2016

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