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Anti-RFC4 Antibody (aa287-336) LS-C102207

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RFC4 Antibodies

Anti-RFC4 Antibody (clone 4H9) LS-C53401
Mouse Monoclonal [clone 4H9] (IgG2b) to Human RFC4
Human
Western blot
Unconjugated
Anti-RFC4 Antibody LS-C90978
Goat Polyclonal to Human RFC4
Human
Western blot, ELISA
Unconjugated
Anti-RFC4 Antibody LS-C90981
Rabbit Polyclonal to Human RFC4
Human
Western blot, Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RFC4
Human, Mouse, Rat, Bat, Dog, Horse, Xenopus
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RFC4
Rabbit
Human, Mouse, Rat, Bat, Dog, Horse, Xenopus (tested or 100% immunogen sequence identity)
Monkey, Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

RFC4 antibody was raised against synthetic peptide located between aa287-336 of human RFC4 (P35249, NP_002907). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Bat, Horse, Xenopus (100%); Marmoset, Panda, Bovine, Rabbit, Pig, Opossum, Platypus (92%); Monkey, Hamster, Elephant, Dog, Guinea pig (85%).
aa287-336
Human RFC4
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RFC4

P35249 BC024022 NP_002907.1

RFC4 Antibody, Activator 1 subunit 4 Antibody, A1 37 kDa subunit Antibody, Activator 1 37 kd subunit Antibody, Activator 1 37 kDa subunit Antibody, RFC37 Antibody, Replication factor C subunit 4 Antibody, RF-C 37 kDa subunit Antibody, RFC 37 kDa subunit Antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits.

Western blot

Western blot
RFC4 antibody LS-C102207 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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