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Anti-RFC4 Antibody (aa287-336) LS-C102207

Catalog Size Price
LS-C102207-50 50 µg (1 mg/ml) Unavailable

Related Products

20 RFC4 Antibodies

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Anti-RFC4 Antibody (aa287-336) LS-C102207
Rabbit Polyclonal (IgG) to Human RFC4
Human, Mouse, Rat, Bat, Dog, Horse, Xenopus
Western blot
Unconjugated
RFC4 antibody LS-C102207 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RFC4 Antibody (clone 1C12) LS-C133089
Mouse Monoclonal [clone 1C12] (IgG) to Human RFC4
Human
IHC, IHC - Paraffin, Western blot, ELISA
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Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Anti-RFC4 Antibody LS-C334086
Rabbit Polyclonal (IgG) to Human RFC4
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, Immunoprecipitation
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Immunohistochemistry of paraffin-embedded rat testis.
Anti-RFC4 Antibody (clone 1A8) LS-C338065
Mouse Monoclonal [clone 1A8] (IgG1) to Human RFC4
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
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IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Anti-RFC4 Antibody LS-C482454
Rabbit Polyclonal to Human RFC4
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of RFC4 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human RFC4
Human, Mouse, Rat, Bat, Dog, Horse, Xenopus
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RFC4
Rabbit
Human, Mouse, Rat, Bat, Dog, Horse, Xenopus (tested or 100% immunogen sequence identity)
Monkey, Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

RFC4 antibody was raised against synthetic peptide located between aa287-336 of human RFC4 (P35249, NP_002907). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Bat, Horse, Xenopus (100%); Marmoset, Panda, Bovine, Rabbit, Pig, Opossum, Platypus (92%); Monkey, Hamster, Elephant, Dog, Guinea pig (85%).
aa287-336
Human RFC4
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About RFC4

P35249 BC024022 NP_002907.1

RFC4 Antibody, Activator 1 subunit 4 Antibody, A1 37 kDa subunit Antibody, Activator 1 37 kd subunit Antibody, Activator 1 37 kDa subunit Antibody, RFC37 Antibody, Replication factor C subunit 4 Antibody, RF-C 37 kDa subunit Antibody, RFC 37 kDa subunit Antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits.

Western blot

RFC4 antibody LS-C102207 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RFC4 antibody LS-C102207 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/26/2018

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