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Anti-RFC3 Antibody (C-Terminus) LS-C80819

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RFC3 Antibodies

Anti-RFC3 Antibody (aa178-227) IHC-plus™ LS-B12568
Rabbit Polyclonal (IgG) to Human RFC3
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RFC3
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RFC3
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Bat, Guinea pig, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

RFC3 antibody was raised against synthetic peptide from C-Terminus of human RFC3 (P40938, NP_002906). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Smelt (100%); Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (92%).
LS-E16796 - Lyophilized - 100 µg - $145.00
C-Terminus
Human RFC3
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RFC3

P40938 NM_002915 NP_002906.1

RFC3 Antibody, Activator 1 38 kDa subunit Antibody, A1 38 kDa subunit Antibody, Activator 1 subunit 3 Antibody, Replication factor C subunit 3 Antibody, RF-C 38 kDa subunit Antibody, RFC, 38 kD subunit Antibody, RFC38 Antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.

Western blot

Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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