Target
Human RBL2 / RB2 p130
Synonyms
RBL2 | p130 | Retinoblastoma-like 2 (p130) | RBR-2 | Rb2 | Retinoblastoma-like protein 2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Immunogen
Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Antiserum will specifically react with a 130 kDa Rb2 protein from human, rat and mouse tissue. No reaction was observed against other related tumor supressor proteins. Cross reactivity with Rb2 (p130) from other species may also occur.
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B414 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B414 was determined to be 1:500.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee