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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RBL2 / RB2 p130 Antibody (C‑Terminus) IHC‑plus™ LS‑B414 Best Seller
Note: This antibody replaces LS-C18885, LS-C17613
RB2 p130 antibody LS-B414 is an unconjugated rabbit polyclonal antibody to RB2 p130 (RBL2) from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B414-50
50 µl (85 mg/ml)
Unavailable

Popular RBL2 / RB2 p130 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Formalin-fixed, paraffin-embedded rat spleen stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of splenic cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
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Product Description

RB2 p130 antibody LS-B414 is an unconjugated rabbit polyclonal antibody to RB2 p130 (RBL2) from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About RBL2 / RB2 p130
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Q08999 NM_005611 NP_005602.3

RBL2 Antibody, p130 Antibody, Retinoblastoma-like 2 (p130) Antibody, RBR-2 Antibody, Rb2 Antibody, Retinoblastoma-like protein 2 Antibody


Specifications

Target
Human RBL2 / RB2 p130
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
C-Terminus
Specificity
Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Immunohistochemistry: LS-B414 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B414 was determined to be 1:500.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.
Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.

Immunohistochemistry

Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.
Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.

Immunohistochemistry

Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.
Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.

Immunohistochemistry

Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.
Anti-RBL2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B414 dilution 1:500.


Requested From: 
Date Requested: 6/18/2018

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