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Anti-RBFOX3 / NEUN Antibody (clone 1B7) LS-C204518

Note: This antibody replaces LS-C180208

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Related Products

Most Popular RBFOX3 / NEUN Antibodies

Anti-RBFOX3 / NEUN Antibody IHC-plus™ LS-B10249
Rabbit Polyclonal to Human RBFOX3 / NEUN
Human, Mouse, Bovine
IHC - Paraffin, ICC
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1B7] (IgG2a,k) to Human RBFOX3 / NEUN
Human, Mouse, Rat, Bovine
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human RBFOX3 / NEUN
Mouse
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1B7]
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:1000 - 1:2000)

Specificity and Use

RBFOX3 / NEUN antibody was raised against n-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Clone 1B7 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.
The antibody solution can be used at dilutions of 1:1000 or higher in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000-1:2000.

Packaging

PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About RBFOX3 / NEUN

A6NFN3 NP_001076044.1

RBFOX3 Antibody

RNA-binding protein that regulates alternative splicing events.

Immunocytochemistry

Immunocytochemistry
Rat brain neural cultures stained with RBFOX3 / NEUN antibody (red), chicken polyclonal antibody to GFAP (green) and DNA (blue). The RBFOX3 / NEUN antibody antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells. Note that we have used the same immunogen to generate a rabbit polyclonal antibody to Fox3/NeuN, RPCA-Fox3.

Western blot

Western blot
Blots of crude rat brain extract stained with RBFOX3 / NEUN antibody. Fox3/NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa.

Requested From: United States
Date Requested: 12/8/2016

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