Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1B7] (IgG2a,k) to Human RBFOX3 / NEUN
Human, Mouse, Rat, Bovine
ICC, Immunofluorescence, Western blot
Human RBFOX3 / NEUN
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1B7]
Western blot (1:1000 - 1:2000)
Specificity and Use
RBFOX3 / NEUN antibody was raised against n-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Clone 1B7 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.
The antibody solution can be used at dilutions of 1:1000 or higher in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000-1:2000.
PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Rat brain neural cultures stained with RBFOX3 / NEUN antibody (red), chicken polyclonal antibody to GFAP (green) and DNA (blue). The RBFOX3 / NEUN antibody antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells. Note that we have used the same immunogen to generate a rabbit polyclonal antibody to Fox3/NeuN, RPCA-Fox3.
Blots of crude rat brain extract stained with RBFOX3 / NEUN antibody. Fox3/NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa.
Requested From: United States
Date Requested: 2/19/2017