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Anti-RASA1 Antibody (aa1034-1047) LS-C38761

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Wt. Vol. Conc. Price
- 100 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular RASA1 Antibodies

Anti-RASA1 Antibody (aa966-993, APC) LS-C239753
Rabbit Polyclonal (IgG) to Human RASA1
Human
Western blot, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RASA1
Human, Monkey, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated

Details

Human RASA1
Rabbit
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • Western blot
  • Immunoprecipitation

Specificity and Use

RASA1 antibody was raised against synthetic peptide corresponding to aa 1034-1047 of human RasGAP (Ras-specific GTPase-activating protein).
aa1034-1047
Recognizes human, mouse, rat and monkey full-length RasGAP as well as the C-terminal (CT) fragment generated by caspase cleavage (RasGAP aa 456-1047). Detects a band of ~150kD by Western blot. Does not cross-react with the N-terminal portion of RasGAP (including the SH2 and SH3 domains).
Immunoprecipitation (1:1000; only CT RasGAP; see technical note). Western Blot (1:1000-1:3000). RasGAP (Ras-specific GTPase-activating protein) is one of the few signalling proteins cleaved by caspases during apoptosis [1-3]. The kinetics of RasGAP cleavage is identical to well-known caspase substrates such as PARP or the caspase themselves. RasGAP cleavage by caspases generates a carboxy-terminal fragment of about 64kD (fragment C) containing the GAP activity and a N-terminal fragment (fragment N) of about 56kD. The latter fragment can be further cleaved into two other smaller fragments, one of which contains the SH2 and SH3 domains. The fact that RasGAP is an abundant ubiquitous protein readily cleaved during apoptosis makes it a very good apoptotic marker. RasGAP is a unique caspase substrate because it can either inhibit or promote apoptosis depending on the extent of its cleavage by caspases. At low levels of caspase activity, generation of fragment N induces a potent Ras-PI3K-Akt-dependent survival pathway. At higher levels of caspase activity, fragment N is further cleaved into two fragments (N1 and N2) that potentiate apoptosis. RasGAP has recently been qualified as an "apoptostat" because it functions as a sensor of caspase activity to determine whether or not a cell should survive [2].

Packaging

For research use only.

About RASA1

P20936 NM_002890 NP_002881.1

RASA1 Antibody, CM-AVM Antibody, CMAVM Antibody, GAP Antibody, GTPase-activating protein Antibody, PKWS Antibody, RASGAP Antibody, Ras p21 protein activator 1 Antibody, Ras p21 protein activator Antibody, p120GAP Antibody, p120RASGAP Antibody, RASA Antibody

RASA1 is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation.

Requested From: United States
Date Requested: 12/10/2016

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