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Anti-Raptor / Mip1 Antibody IHC-plus™ LS-B579

Note: This antibody replaces LS-C19502, LS-C131798
Catalog Size Price
LS-B579-50 50 µg (1 mg/ml) Unavailable

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Anti-Raptor / Mip1 Antibody IHC-plus™ LS-B579
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Immunohistochemistry Image
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Anti-Raptor / Mip1 Antibody (aa101-150) IHC-plus™ LS-B3798
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Anti-Raptor / Mip1 Antibody (aa900-950) IHC-plus™ LS-B6513
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Anti-Raptor / Mip1 Antibody (aa1-50) IHC-plus™ LS-B14478
Rabbit Polyclonal (IgG) to Human Raptor / Mip1
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100% Guaranteed
Rabbit Polyclonal to Human Raptor / Mip1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human Raptor / Mip1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Western blot (2 µg/ml)

Specificity and Use

Raptor / Mip1 antibody was raised against synthetic peptide from human RPTOR / Raptor.
LS-E30134 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B579. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
15 amino acid peptide from the central region of human Raptor
Immunohistochemistry: LS-B579 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B579 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About Raptor / Mip1

Q8N122 NM_020761 NP_065812.1

RPTOR Antibody, KOG1 Antibody, KIAA1303 Antibody, RAPTOR Antibody, Mip1 Antibody

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids.

Immunohistochemistry

Immunohistochemistry
Anti-RPTOR / Raptor antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-RPTOR / Raptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1ug/ml

Immunofluorescence

Immunofluorescence
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 ug/ml.

Western blot

Western blot
Western blot of Raptor in L1210 cell lysate with LS-B579 at (A) 2 and (B) 4 ug/ml.

Requested From: 
Date Requested: 6/23/2017

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