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Anti-Raptor / Mip1 Antibody IHC-plus™ LS-B579

Note: This antibody replaces LS-C19502, LS-C131798
Catalog Size Price
LS-B579-50 50 µg (1 mg/ml) Unavailable

Related Products

84 Raptor / Mip1 Antibodies

Most Popular Raptor / Mip1 Antibodies

Anti-Raptor / Mip1 Antibody IHC-plus™ LS-B579
Rabbit Polyclonal (IgG) to Human Raptor / Mip1
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
Anti-RPTOR / Raptor antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.
Anti-Raptor / Mip1 Antibody (phospho-Ser863) LS-C96828
Rabbit Polyclonal to Human Raptor / Mip1
Human
Dot Blot
Unconjugated
Dot blot of anti-raptor-pS863 antibody (RB13350) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Anti-Raptor / Mip1 Antibody (aa101-150) IHC-plus™ LS-B3798
Rabbit Polyclonal (IgG) to Human Raptor / Mip1
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
Anti-RPTOR / Raptor antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3798 concentration 5 ug/ml.
Anti-Raptor / Mip1 Antibody (aa900-950) IHC-plus™ LS-B6513
Rabbit Polyclonal to Human Raptor / Mip1
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-RPTOR / Raptor antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6513 dilution 1:100.
Anti-Raptor / Mip1 Antibody (clone 1411CT316.2.151.34) LS-C344171
Mouse Monoclonal [clone 1411CT316.2.151.34] (IgG1,k) to Human Raptor / Mip1
Human, Mouse, Rat
Western blot
Unconjugated
Western blot of lysates from HeLa, MCF-7, mouse C2C12, mouse NIH/3T3, rat C6 cell line (from left to right) with RPTOR Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human Raptor / Mip1
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human Raptor / Mip1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Western blot (2 µg/ml)

Specificity and Use

LS-E30134 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B579. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunohistochemistry: LS-B579 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B579 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
For research use only.

About Raptor / Mip1

Q8N122 NM_020761 NP_065812.1

RPTOR Antibody, KOG1 Antibody, KIAA1303 Antibody, RAPTOR Antibody, Mip1 Antibody

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids.

Immunohistochemistry

Anti-RPTOR / Raptor antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.
Anti-RPTOR / Raptor antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.

Immunohistochemistry

Anti-RPTOR / Raptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.
Anti-RPTOR / Raptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B579 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1µg/mL
Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1µg/mL

Immunocytochemistry

Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1ug/ml
Immunocytochemistry staining of L1210 cells using RAPTOR antibody at 1ug/ml

Immunofluorescence

Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 µg/mL.
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 µg/mL.

Immunofluorescence

Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 ug/ml.
Immunofluorescence of Raptor in L1210 cells with Raptor antibody at 10 ug/ml.

Western blot

Western blot analysis of Raptor in L1210 cell lysate with Raptor (IN) antibody at (A) 2 and (B) 4 µg/mL.
Western blot analysis of Raptor in L1210 cell lysate with Raptor (IN) antibody at (A) 2 and (B) 4 µg/mL.

Western blot

Western blot of Raptor in L1210 cell lysate with LS-B579 at (A) 2 and (B) 4 ug/ml.
Western blot of Raptor in L1210 cell lysate with LS-B579 at (A) 2 and (B) 4 ug/ml.

Requested From: 
Date Requested: 5/28/2018

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