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Anti-RANGAP1 Antibody IHC-plus™ LS-B2883

Note: This antibody replaces LS-C82248, LS-C93628

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RANGAP1 Antibodies

Anti-RANGAP1 Antibody IHC-plus™ LS-B2883
Rabbit Polyclonal to Xenopus RANGAP1
Xenopus, Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RANGAP1 Antibody (aa20-49, APC) LS-C267819
Rabbit Polyclonal (IgG) to Human RANGAP1
Human
IHC, Western blot, ELISA
APC Conjugated
Anti-RANGAP1 Antibody (aa20-49, Azide-free, HRP) LS-C267822
Rabbit Polyclonal (IgG) to Human RANGAP1
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Xenopus RANGAP1
Xenopus, Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Xenopus RANGAP1
Rabbit
Xenopus, Human (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)

Specificity and Use

RANGAP1 antibody was raised against a mixture of two peptides from Xenopus RanGAP, n-CHWSDMFTGRLRPEI-c & n-CPSPEKLVRMGPRRSA-. Percent identity by BLAST analysis: Xenopus (100%); Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Zebrafish (93%); Seabass, Salmon, Stickleback, Pufferfish (87%); Nematode (80%).
Reacts with human & Xenopus RanGAP
Immunohistochemistry: LS-B2883 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2883 was determined to be 1:100.

Packaging

PBS, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RANGAP1

P46060 NM_002883 NP_002874.1

RANGAP1 Antibody, Fug1 Antibody, KIAA1835 Antibody, SD Antibody, Segregation distortion Antibody, Segregation distorter homolog Antibody

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p.

Immunohistochemistry

Immunohistochemistry
Anti-RANGAP1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2883 dilution 1:100.

Immunohistochemistry

Immunohistochemistry
Anti-RANGAP1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2883 dilution 1:100.

Requested From: United States
Date Requested: 12/10/2016

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