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Anti-RAD52 Antibody (clone 5H9) IHC-plus™ LS-B1709

Note: This antibody replaces LS-C20095

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAD52 Antibodies

Anti-RAD52 Antibody (aa360-375) IHC-plus™ LS-B413
Rabbit Polyclonal to Human RAD52
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RAD52 Antibody (aa150-200) LS-C285967
Rabbit Polyclonal (IgG) to Human RAD52
Human, Dog, Guinea pig, Pig, Rabbit
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5H9] (IgG) to Human RAD52
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human RAD52
Mouse
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [5H9]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

RAD52 antibody was raised against recombinant human RAD52.
Human Rad52 recombinant protein expressed in E. coli.
Immunohistochemistry: LS-B1709 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1709 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2, Contains no preservative.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About RAD52

P43351 AF187983 NP_602296.2

RAD52 Antibody, RAD52 (S. cerevisiae) homolog Antibody, RAD52 homolog (S. cerevisiae) Antibody, Recombination protein RAD52 Antibody

RAD52 shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2.

Immunohistochemistry

Immunohistochemistry
Anti-RAD52 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1709 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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