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Anti-RAD51L3 / RAD51D Antibody IHC-plus™ LS-B320

Note: This antibody replaces LS-C2864

Ordering

Wt. Vol. Conc. Price
- 100 µl 1.9 mg/ml Unavailable

Most Popular RAD51L3 / RAD51D Antibodies

Anti-RAD51L3 / RAD51D Antibody IHC-plus™ LS-B320
Rabbit Polyclonal to Human RAD51L3 / RAD51D
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RAD51L3 / RAD51D Antibody (Biotin) LS-C140846
Rabbit Polyclonal to Human RAD51L3 / RAD51D
Human
IHC, IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image
Anti-RAD51L3 / RAD51D Antibody (DY650) LS-C140847
Rabbit Polyclonal to Human RAD51L3 / RAD51D
Human
IHC, IHC - Paraffin, Western blot
DY650 Conjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human RAD51L3 / RAD51D
Human
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RAD51L3 / RAD51D
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:1000)
  • (applications tested for the base form of this product only)

Specificity and Use

RAD51L3 / RAD51D antibody was raised against a peptide from human RAD51D (homologue 4)
HRAD51D. There is no cross-reactivity observed with XRCC2, XRCC3, RAD51, RAD51B, or RAD51C.
Immunohistochemistry: LS-B320 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B320 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RAD51L3 / RAD51D

O75771 NM_002878 NP_002869.3

RAD51D Antibody, BROVCA4 Antibody, HsTRAD Antibody, RAD51 homolog D Antibody, RAD51-like protein 3 Antibody, Recombination repair protein Antibody, R51H3 Antibody, RAD51L3 Antibody, RAD51 (S. cerevisiae)-like 3 Antibody, RAD51-like 3 (S. cerevisiae) Antibody, TRAD Antibody

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment.

Immunohistochemistry

Immunohistochemistry
Anti-RAD51L3 / RAD51D antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B320 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/28/2017

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