Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
RAB4A / RAB4 is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.
Western Blot analysis of RAB4A expression in transfected 293T cell line by RAB4A monoclonal antibody (M01), clone 1C10.Lane 1: RAB4A transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.
RAB4A monoclonal antibody (M01), clone 1C10 Western Blot analysis of RAB4A expression in HeLa.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RAB4A is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis of RAB4A over-expressed 293 cell line, cotransfected with RAB4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB4A monoclonal antibody (M01), clone 1C10 . GAPDH ( 36.1 kDa ) used as specificity and loading control.