Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 6A8.6F10.1A6] (IgG1,k) to Mouse Qa-1b
Western blot, Immunoprecipitation, Flow Cytometry, Neutralization
Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [6A8.6F10.1A6]
Protein G purified
Western blot (1 - 2 µg/ml)
Specificity and Use
Qa-1b antibody was raised against animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the Qa-1b Class Ib molecule.
Flow: Cell Surface.
PBS, 0.05% BSA, 0.05% sodium azide
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
Western blot of Qa-1b in mouse thymus tissue lysate using LS-C148391 at 2 ug/ml.
Flow cytometry of Qa-1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) LS-C148391 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells.
Flow cytometry of Qa-1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) LS-C148391 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in the entire population of cells.