Target
Human PTPN6 / SHP1
Synonyms
PTPN6 | HCP | HPTP1C | Hematopoietic cell phosphatase | PTP-1C | SH-PTP1 | SHP-1L | SHP-1 | Shptp1 | HCPH | PTP1C | SHP1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-HTKNKREEKVKKQ, from the internal region (near the C-Terminus) of the protein sequence according to NP_536858.1NP_002822.2.
Specificity
Human PTPN6. This antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2)
Applications
- Western blot (0.2 - 0.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70 kDa band observed in lysates of cell lines Jurkat and U937 (calculated MW of 67.7 kDa according to NP_536858.1 and 67.6 kDa according to NP_002822.2). Recommended concentration: 0.2-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee