Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone SPM209] (IgG2a,k) to Human PTHR / PTHR1
IHC - Paraffin, Western blot
Human PTHR / PTHR1
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [SPM209]
Protein A purified
IHC - Paraffin (1:50)
Western blot (1 - 2 µg/ml)
Specificity and Use
PTHR / PTHR1 antibody was raised against synthetic peptide corresponding to 23aa of the hPTH/PTHrP receptor, which is not shared by the type II receptor.
Human Parathyroid Hormone Receptor Type 1
Immunohistochemistry (formalin fixed paraffin embedded): 1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months.
PTHR / PTHR1 is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis.