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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMA5 Antibody (aa106‑135) LS‑C98175
PSMA5 antibody LS-C98175 is an unconjugated rabbit polyclonal antibody to human PSMA5. Validated for IHC and WB.
Catalog
Size
Price
LS-C98175-400
400 µl
Unavailable

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Antibody
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Product Description

PSMA5 antibody LS-C98175 is an unconjugated rabbit polyclonal antibody to human PSMA5. Validated for IHC and WB.
About PSMA5
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. P28066 NM_002790 NP_002781.2

PSMA5 Antibody, Proteasome component 5 Antibody, Proteasome subunit zeta Antibody, Proteasome alpha 5 subunit Antibody, Macropain zeta chain Antibody, Zeta Antibody, Proteasome zeta chain Antibody, Macropain subunit zeta Antibody, Proteasome alpha-type 5 Antibody, PSC5 Antibody


Specifications

Target
Human PSMA5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E3329 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98175. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa106-135
Specificity
This PSMA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the Central region of human PSMA5.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).
Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).

Western blot

Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.
Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).
Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).

Western blot

Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.
Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).
Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).

Western blot

Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.
Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).
Western blot of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) (Origene Technologies).

Western blot

Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.
Western blot of PSMA5 Antibody in HL60 cell line lysates (35 ug/lane). PSMA5(arrow) was detected using the purified polyclonal antibody.


Requested From: 
Date Requested: 6/20/2018

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