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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRPF6 / TOM Antibody (aa851‑900) LS‑C40319
TOM antibody LS-C40319 is an unconjugated rabbit polyclonal antibody to TOM (PRPF6) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C40319-100
100 µg
$325

Popular PRPF6 / TOM Products

Detection of Human PRP6 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:200 (1 Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Detection of Human PRP6 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:1000 (0.2 Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of PRPF6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, ELISA
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PRPF6 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

TOM antibody LS-C40319 is an unconjugated rabbit polyclonal antibody to TOM (PRPF6) from human, mouse, rat and other species. Validated for WB.
About PRPF6 / TOM
Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. O94906 NM_012469 NP_036601.2

PRPF6 Antibody, ANT1 Antibody, C20orf14 Antibody, BB152O15.1 Antibody, HPrp6 Antibody, Prp6 Antibody, PRP6 homolog Antibody, TOM Antibody, U5-102K Antibody, Pre-mRNA-processing factor 6 Antibody, ANT-1 Antibody, U5-102 kDa protein Antibody


Specifications

Target
Human PRPF6 / TOM
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Guinea pig, Horse, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (2.5 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PRPF6 / TOM antibody was raised against synthetic peptide located between aa851-900 of human PRPF6 (O94906, NP_036601). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Stickleback (100%); Orangutan, Rabbit, Xenopus (92%); Dog, Bovine, Horse, Platypus, Zebrafish (85%).
Epitope
aa851-900
Specificity
Human PRPF6
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PRPF6 antibody LS-C40319 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/19/2018

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