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Anti-PRMT4 / CARM1 Antibody (aa209-379) LS-C149891

Catalog Size Price
LS-C149891-25 25 µg Unavailable
LS-C149891-100 100 µg Unavailable

Related Products

55 PRMT4 / CARM1 Antibodies

Most Popular PRMT4 / CARM1 Antibodies

Anti-PRMT4 / CARM1 Antibody (N-Terminus) LS-C137169
Rabbit Polyclonal (IgG) to Human PRMT4 / CARM1
Human, Mouse, Rat
IHC, Western blot, ELISA
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Anti-PRMT4 / CARM1 Antibody (N-Terminus) IHC-plus™ LS-B10076
Rabbit Polyclonal (IgG) to Human PRMT4 / CARM1
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IHC - Paraffin, Immunofluorescence, Western blot, ELISA
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Anti-PRMT4 / CARM1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10076 concentration 10 ug/ml.
Anti-PRMT4 / CARM1 Antibody (aa450-500) LS-C287314
Rabbit Polyclonal (IgG) to Human PRMT4 / CARM1
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
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Detection of Human CARM1/PRMT4 by Immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-CARM1/PRMT4 used at a dilution of 1:1000 (1 ug/ml).
Anti-PRMT4 / CARM1 Antibody (C-Terminus) IHC-plus™ LS-B11297
Rabbit Polyclonal (IgG) to Human PRMT4 / CARM1
Human, Mouse, Rat, Bovine, Dog, Pig
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
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Anti-PRMT4 / CARM1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRMT4 / CARM1 Antibody (phospho-Ser228) LS-C335962
Rabbit Polyclonal (IgG) to Human PRMT4 / CARM1
Human, Mouse, Rat
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence staining of methanol-fixed HeLa cells.

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Goat Polyclonal (IgG) to Human PRMT4 / CARM1
Human, Mouse
ICC, Western blot
Unconjugated
Carm1 in HeLa Human Cell Line. Carm1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. Detection of Human Carm1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Carm1 at approximately 63 kD (as indicated). This experiment was conducted under reducing conditions.

Details

Human PRMT4 / CARM1
Goat
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

PRMT4 / CARM1 antibody was raised against e. coli-derived recombinant human Carm1 Lys209-Leu379 Accession # Q86X55.
aa209-379
Detects human Carm1 in direct ELISAs and Western blots.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PRMT4 / CARM1

Q86X55 NM_199141 NP_954592.1

CARM1 Antibody, PRMT4 Antibody

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling.
(More About PRMT4 / CARM1)

Immunocytochemistry

Carm1 in HeLa Human Cell Line. Carm1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm.
Carm1 in HeLa Human Cell Line. Carm1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm.

Western blot

Detection of Human Carm1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Carm1 at approximately 63 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of Human Carm1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human Carm1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Carm1 at approximately 63 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: 
Date Requested: 4/20/2018
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