Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
PRKCI Antibody, APKC-lambda/iota Antibody, DXS1179E Antibody, PKC iota Antibody, Protein kinase C, iota Antibody, Protein kinase C iota type Antibody, NPKC-iota Antibody, PKCI Antibody, PRKC-lambda/iota Antibody, Protein kinase C iota Antibody
Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis.
Immunofluorescence of monoclonal antibody to PRKCI on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody (M01), clone 1G11.Lane 1: PRKCI transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PRKCI is approximately 3 ng/ml as a capture antibody.
Western blot analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCI monoclonal antibody (M01) clone 1G11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.