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Anti-PRKCI / PKC Iota Antibody (clone 1G11) LS-C197989

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular PRKCI / PKC Iota Antibodies

Anti-PRKCI / PKC Iota Antibody (N-Terminus, AP) LS-C281443
Rabbit Polyclonal (IgG) to Human PRKCI / PKC Iota
Human, Mouse
IHC, Western blot, ELISA
AP Conjugated
Anti-PRKCI / PKC Iota Antibody (N-Terminus, FITC) LS-C281446
Rabbit Polyclonal (IgG) to Human PRKCI / PKC Iota
Human, Mouse
IHC, Western blot, ELISA
FITC Conjugated
Anti-PRKCI / PKC Iota Antibody (aa21-214) LS-C357506
Rabbit Polyclonal (IgG) to Human PRKCI / PKC Iota
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Mouse Monoclonal [clone 1G11] (IgG1,k) to Human PRKCI / PKC Iota
Human
Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated

Details

Human PRKCI / PKC Iota
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1G11]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

PRKCI / PKC Iota antibody was raised against pRKCI (AAH22016, aa1-587 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PRKCI / PKC Iota
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About PRKCI / PKC Iota

P41743 NM_002740 NP_002731.4

PRKCI Antibody, APKC-lambda/iota Antibody, DXS1179E Antibody, PKC iota Antibody, Protein kinase C, iota Antibody, Protein kinase C iota type Antibody, NPKC-iota Antibody, PKCI Antibody, PRKC-lambda/iota Antibody, Protein kinase C iota Antibody

Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PRKCI on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody (M01), clone 1G11.Lane 1: PRKCI transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PRKCI is approximately 3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCI monoclonal antibody (M01) clone 1G11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: United States
Date Requested: 12/7/2016

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