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Anti-PRKAB1 / AMPK Beta 1 Antibody (aa147-196) IHC-plus™ LS-B6991

Note: This antibody replaces LS-C117592
Catalog Size Price
LS-B6991-50 50 µl Unavailable

Most Popular PRKAB1 / AMPK Beta 1 Antibodies

Anti-PRKAB1 / AMPK Beta 1 Antibody (aa147-196) IHC-plus™ LS-B6991
Rabbit Polyclonal (IgG) to Human PRKAB1 / AMPK Beta 1
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-PRKAB1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6991 dilution 1:100.
Anti-PRKAB1 / AMPK Beta 1 Antibody (N-Terminus) LS-C354056
Rabbit Polyclonal to Human PRKAB1 / AMPK Beta 1
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of AMPK beta 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-PRKAB1 / AMPK Beta 1 Antibody (aa10-90) LS-C382017
Rabbit Polyclonal (IgG) to Human PRKAB1 / AMPK Beta 1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-PRKAB1 / AMPK Beta 1 Antibody LS-C393619
Mouse Monoclonal to Human PRKAB1 / AMPK Beta 1
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse monoclonal antibody (1:200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Anti-PRKAB1 / AMPK Beta 1 Antibody LS-C408251
Mouse Monoclonal (IgG) to Human PRKAB1 / AMPK Beta 1
Human, Mouse, Rat
IHC, ICC, Western blot, Immunoprecipitation
Unconjugated
Western blot analysis of extracts of various cells.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human PRKAB1 / AMPK Beta 1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PRKAB1 / AMPK Beta 1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)
  • ELISA (1:5000)

Specificity and Use

Synthetic peptide from human AMPK beta1 (aa147-196).
aa147-196
PRKAB1 antibody detects endogenous levels of total AMPK beta1 protein.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About PRKAB1 / AMPK Beta 1

Q9Y478 NM_006253 NP_006244.2

PRKAB1 Antibody, AMPK beta 1 Antibody, AMPK subunit beta-1 Antibody, AMPKb Antibody, AMPK Antibody, AMPK beta -1 chain Antibody, HAMPKb Antibody

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation.

Immunohistochemistry

Anti-PRKAB1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6991 dilution 1:100.
Anti-PRKAB1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6991 dilution 1:100.

Western blot

Western blot of extracts from HepG2/Jurkat/HUVEC/MCF-7 cells, using PRKAB1 antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HepG2/Jurkat/HUVEC/MCF-7 cells, using PRKAB1 antibody. The lane on the right is treated with the synthesized peptide.

Requested From: 
Date Requested: 4/20/2018

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