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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP1R1B / DARPP‑32 Antibody (phospho‑Thr75) LS‑C27
Note: This antibody replaces LS-C54357, LS-C49254, LS-C64512, LS-C83797, LS-C64516, LS-C22758, LS-C22757, LS-C22763, LS-C48594, LS-C43072
DARPP-32 antibody LS-C27 is an unconjugated rabbit polyclonal antibody to DARPP-32 (PPP1R1B) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C27-100
100 µl
Unavailable

Popular PPP1R1B / DARPP-32 Products

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with l-Ptase.
Species: Rat, Human, Mouse, Bovine, Dog, Chicken
Applications: IHC, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, Western blot

Product Description

DARPP-32 antibody LS-C27 is an unconjugated rabbit polyclonal antibody to DARPP-32 (PPP1R1B) from human, mouse, rat and other species. Validated for IHC and WB.
About PPP1R1B / DARPP-32
PPP1R1B / DARPP-32 is a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. Q9UD71 NM_032192 NP_115568.2

PPP1R1B Antibody, DARPP-32 Antibody, DARPP32 Antibody


Specifications

Target
Rat PPP1R1B / DARPP-32
Host
Rabbit
Reactivity
Rat, Human, Mouse, Bovine, Dog, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:25)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr7 of rat DARPP-32.
Epitope
pThr75
Specificity
Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by a,-phosphatase treatment
Presentation
50 uL of 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50% glycerol.
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical

Western blot

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical

Western blot

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical

Western blot

Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda phosphatase). The blot is identical

Requested From: 
Date Requested: 7/17/2018

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