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Anti-PPP1R13L / iASPP Antibody (aa780-797) IHC-plus™ LS-B408

Note: This antibody replaces LS-C19094

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPP1R13L / iASPP Antibodies

Anti-PPP1R13L / iASPP Antibody (aa780-797) IHC-plus™ LS-B408
Rabbit Polyclonal to Human PPP1R13L / iASPP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PPP1R13L / iASPP Antibody (aa75-125) IHC-plus™ LS-B5248
Rabbit Polyclonal (IgG) to Human PPP1R13L / iASPP
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-PPP1R13L / iASPP Antibody (aa756-805) IHC-plus™ LS-B11881
Rabbit Polyclonal to Human PPP1R13L / iASPP
Human, Mouse, Rat, Dog, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PPP1R13L / iASPP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human PPP1R13L / iASPP
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:40000 - 1:160000)

Specificity and Use

PPP1R13L / iASPP antibody was raised against synthetic peptide from human PPP1R13L / IASPP.
aa780-797
Amino acids 780-797 of human iASPP (isoform 1) protein.
Immunohistochemistry: LS-B408 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B408 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About PPP1R13L / iASPP

Q8WUF5 NM_006663 NP_006654.2

PPP1R13L Antibody, IASPP Antibody, Inhibitor of ASPP protein Antibody, NFkB-interacting protein 1 Antibody, NKIP1 Antibody, RAI4 Antibody, RAI Antibody, Retinoic acid induced 4 Antibody, NFkB interacting protein 1 Antibody, PPP1R13BL Antibody, Protein iASPP Antibody, RelA-associated inhibitor Antibody, PPP1R13B-like protein Antibody

Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-PPP1R13L / IASPP antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B408 concentration 5 ug/ml.

Western blot

Western blot
Anti-iASPP Antibody - Western Blot. Western blot of affinity purified anti-iASPP antibody shows detection of a band at ~100 kD (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/9/2016

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