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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP1CB Antibody (aa298‑327) LS‑C162076
PPP1CB antibody LS-C162076 is an unconjugated rabbit polyclonal antibody to human PPP1CB. Validated for WB.
Catalog
Size
Price
LS-C162076-400
400 µl
Unavailable

Popular PPP1CB Products

Antibody
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Detection of Human PPP1CB by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PPP1CB used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Dog, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of PP1 beta staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human esophageal cancer tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

PPP1CB antibody LS-C162076 is an unconjugated rabbit polyclonal antibody to human PPP1CB. Validated for WB.
About PPP1CB
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. P62140 NM_002709 NP_002700.1

PPP1CB Antibody, PP-1B Antibody, pp1 delta Antibody, PPP1CD Antibody, Protein phosphatase 1-beta Antibody, Protein phosphatase 1-delta Antibody, PP1beta Antibody, PP1 beta Antibody


Specifications

Target
Human PPP1CB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Rabbit, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E2776 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162076. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa298-327
Specificity
This PPP1CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the C-terminal region of human PPP1CB.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Western blot

Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Western blot

Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Western blot

Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Western blot of lysates from HT-29, PC-12 cell line and mouse brain tissue lysate (from left to right), using PPP1CB Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.


Requested From: 
Date Requested: 6/25/2018

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