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Anti-PPM1B Antibody (clone 1A3-2A4) LS-C197966

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPM1B Antibodies

Anti-PPM1B Antibody (C-Terminus) LS-C91551
Rabbit Polyclonal to Human PPM1B
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-PPM1B Antibody (aa1-479) LS-C131253
Rabbit Polyclonal to Human PPM1B
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1A3-2A4] (IgG1,k) to Human PPM1B
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA, RNA interference
Unconjugated

Details

Human PPM1B
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1A3-2A4]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference

Specificity and Use

PPM1B antibody was raised against pPM1B (AAH12002, aa1-192 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PPM1B
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About PPM1B

O75688 NM_002706 NP_002697.1

PPM1B Antibody, Phosphatase 2c beta Antibody, PPC2BETAX Antibody, PP2C-beta Antibody, Protein phosphatase 2A beta Antibody, Protein phosphatase 1B Antibody, PP2CB Antibody, PP2CBETA Antibody, PP2C-beta-X Antibody

PPM1B is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PPM1B on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody (M01), clone 1A3-2A4.Lane 1: PPM1B transfected lysate(52.643 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPM1B monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PPM1B is 0.3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PPM1B monoclonal antibody (M01), clone 1A3-2A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
 

Requested From: United States
Date Requested: 12/10/2016

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