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Anti-PPBP / CXCL7 Antibody IHC-plus™ LS-B7394

Note: This antibody replaces LS-C104533

Ordering

Wt. Vol. Conc. Price
50 µg - - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

PPBP / CXCL7 Antibody LS-B7394 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PPBP antibody IHC of human intravascular platelets.
Western blot
Western Blot (non-reducing) of PPBP / CXCL7 antibody LS-B7394
Western blot
Western Blot of PPBP / CXCL7 antibody LS-B7394
Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of PPBP / CXCL7 antibody LS-B7394

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Rabbit Polyclonal to Human PPBP / CXCL7
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human PPBP / CXCL7
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.1 - 0.2 µg/ml), ELISA (0.5 - 2 µg/ml), Neutralization (5 - 10 µg/ml)

Specificity and Use

PPBP / CXCL7 antibody was raised against e.coli derived recombinant Human NAP-2.
Human NAP-2
Immunohistochemistry: LS-B7394 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hNAP-2 (100 ng/ml), a concentration of 5-10 ug/ml of this antibody is required. ELISA: To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NAP-2 (LS-C104604) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Reconstitute in sterile water
Store lyophilized antibody at -20C for up to one year from date of receipt. The reconstituted antibody is stable for at least two weeks at 2-8 C. Frozen aliquots are stable for at least 6 months when stored at -20 C. Avoid repeated freeze-thaw cycles.
For research use only.

About PPBP / CXCL7

P02775 NM_002704 NP_002695.1

PPBP Antibody, Beta-thromboglobulin Antibody, B-TG1 Antibody, Beta-TG Antibody, C-X-C motif chemokine 7 Antibody, CTAP3 Antibody, CXCL7 Antibody, CTAP-III Antibody, CXC chemokine ligand 7 Antibody, NAP-2 Antibody, NAP-2-L1 Antibody, PBP Antibody, LA-PF4 Antibody, LDGF Antibody, SCYB7 Antibody, Small-inducible cytokine B7 Antibody, Small inducible cytokine B7 Antibody, THBGB1 Antibody, Thrombocidin 2 Antibody, TC2 Antibody, TGB Antibody, Thrombocidin 1 Antibody, Thromboglobulin, beta-1 Antibody, CTAPIII Antibody, MDGF Antibody, Platelet basic protein Antibody, SCAR10 Antibody, TC1 Antibody, THBGB Antibody

The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan.

Immunohistochemistry

Immunohistochemistry
Anti-PPBP antibody IHC of human intravascular platelets. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7394 concentration 5 ug/ml.

Western blot

Western blot
Western Blot (non-reducing) of PPBP / CXCL7 antibody LS-B7394

Western blot

Western blot
Western Blot of PPBP / CXCL7 antibody LS-B7394

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of PPBP / CXCL7 antibody LS-B7394

Requested From: United States
Date Requested: 4/26/2015

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