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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPARA / PPAR Alpha Antibody (clone 2E7) LS‑C173299
PPAR Alpha antibody LS-C173299 is an unconjugated mouse monoclonal antibody to human PPAR Alpha (PPARA). Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C173299-100
100 µl (0.68 mg/ml)
Unavailable

Popular PPARA / PPAR Alpha Products

Immunohistochemistry using anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.
Species: Mouse, Human, Rat, Bovine, Dog, Hamster, Boar
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PPAR Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1604 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Dog, Hamster
Applications: IHC, IHC - Paraffin, Western blot
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
PPAR Alpha antibody. IHC(P): Human Breast Cancer Tissue.
Species: Mouse, Human, Rat, Rabbit
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Immunohistochemical of paraffin-embedded H. kidney section using PPARA Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

PPAR Alpha antibody LS-C173299 is an unconjugated mouse monoclonal antibody to human PPAR Alpha (PPARA). Validated for Flow, IF and WB.
About PPARA / PPAR Alpha
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. Q07869 NM_005036 NP_005027.2

PPARA Antibody, PPAR Alpha Antibody, PPARalpha Antibody, PPAR-alpha Antibody, NR1C1 Antibody, HPPAR Antibody, PPAR Antibody


Specifications

Target
Human PPARA / PPAR Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2E7]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:500)
  • Flow Cytometry (1:100)
Immunogen
Human recombinant protein fragment corresponding to amino acids 145-420 of human PPARA(NP_005027) produced in E. coli.
Specificity
Human PPAR Alpha
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.
Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.
Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.
Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.
Anti-PPARA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPARA antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/23/2018

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