Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
POU5F1 Antibody, Oct-4 Antibody, OCT3 Antibody, OCT4 Antibody, OTF4 Antibody, POU class 5 homeobox 1 Antibody, Octamer-binding protein 3 Antibody, Octamer-binding protein 4 Antibody, OTF-3 Antibody, Oct-3 Antibody, OTF3 Antibody
POU5F1 / OCT4 is a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms.
Oct4 antibody (9B7) at 1:5000 dilution + lysates from HEK-293T cell transfected with human OCT4 expression vector.
IHC of paraffin-embedded Human endometrium tissue using anti-OCT4 mouse monoclonal antibody.
Anti-OCT4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OCT4.
Anti-OCT-4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OCT-4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT-4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT-4.