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Anti-POU5F1 / OCT4 Antibody (aa100-200) IHC-plus™ LS-B85

Note: This antibody replaces LS-C2740

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POU5F1 / OCT4 Antibodies

Anti-POU5F1 / OCT4 Antibody (Internal, Biotin) LS-C140990
Rabbit Polyclonal to Human POU5F1 / OCT4
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Biotin Conjugated
Immunohistochemistry Image
Anti-POU5F1 / OCT4 Antibody (clone MRQ-10) LS-C202890
Mouse Monoclonal [clone MRQ-10] (IgG1) to Human POU5F1 / OCT4
Human
IHC - Paraffin, IHC - Frozen
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human POU5F1 / OCT4
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry (applications tested for the base form of this product only)
Unconjugated

Details

Human POU5F1 / OCT4
Rabbit
Human (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (2 µg/ml)
  • Flow Cytometry (1:50 - 1:200)
  • (applications tested for the base form of this product only)

Specificity and Use

POU5F1 / OCT4 antibody was raised against a synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200). Percent identity by BLAST analysis: Bovine, Cat, Porcine (93%).
aa100-200
OCT4 - Embryonic Stem Cell Marker.
Immunohistochemistry: LS-B85 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B85 was determined to be 2.5 ug/ml.

Packaging

Tris-glycine buffer, 150 mM sodium chloride, 0.05% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About POU5F1 / OCT4

Q01860 NM_002701 NP_002692.2

POU5F1 Antibody, Oct-4 Antibody, OCT3 Antibody, OCT4 Antibody, OTF4 Antibody, POU class 5 homeobox 1 Antibody, Octamer-binding protein 3 Antibody, Octamer-binding protein 4 Antibody, OTF-3 Antibody, Oct-3 Antibody, OTF3 Antibody

POU5F1 / OCT4 is a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms.

Immunohistochemistry

Immunohistochemistry
Anti-POU5F1 / OCT4 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B85 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/2/2016

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