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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLR3B Antibody (aa330‑359) LS‑C163856
POLR3B antibody LS-C163856 is an unconjugated rabbit polyclonal antibody to POLR3B from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C163856-400
400 µl
Unavailable

Popular POLR3B Products

Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
Detection of Human RPC2 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPC2 used at a dilution of 1:500.
Species: Human, Bovine, Dog, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human RPC2 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RPC2 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Species: Human, Bovine, Dog, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of POLR3B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

POLR3B antibody LS-C163856 is an unconjugated rabbit polyclonal antibody to POLR3B from human and mouse. Validated for WB.
About POLR3B
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. NM_018082

POLR3B Antibody, HLD8 Antibody, RPC2 Antibody, C128 Antibody, RNA polymerase III subunit C2 Antibody


Specifications

Target
Human POLR3B
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E2732 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C163856. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa330-359
Specificity
This POLR3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-359 amino acids from the N-terminal region of human POLR3B.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).
POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).

Western blot

POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).
POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).

Western blot

POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).
POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).

Western blot

POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).
POLR3B Antibody western blot of mouse spleen tissue lysates (35 ug/lane). The POLR3B antibody detected the POLR3B protein (arrow).


Requested From: 
Date Requested: 6/20/2018

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