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Anti-POLR2J2 Antibody (clone 6E9) LS-C172695

Catalog Size Price
LS-C172695-100 100 µl (0.94 mg/ml) Unavailable

Related Products

3 POLR2J2 Antibodies

Most Popular POLR2J2 Antibodies

Anti-POLR2J2 Antibody (clone 8F10) LS-C172620
Mouse Monoclonal [clone 8F10] (IgG2a) to Human POLR2J2
Human, Monkey, Rat, Dog
Western blot
Unconjugated
Western blot of extracts (35 ug) from 9 different cell lines by using anti-POLR2J2 monoclonal antibody.
Anti-POLR2J2 Antibody (clone 1C6) LS-C172668
Mouse Monoclonal [clone 1C6] (IgG1) to Human POLR2J2
Human
Western blot, Flow Cytometry
Unconjugated
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Anti-POLR2J2 Antibody (clone 6E9) LS-C172695
Mouse Monoclonal [clone 6E9] (IgG2a) to Human POLR2J2
Human
Western blot, Flow Cytometry
Unconjugated
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Anti-POLR2J2 Antibody (clone 3G4) LS-C337263
Mouse Monoclonal [clone 3G4] (IgG2a) to Human POLR2J2
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
IHC of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

100% Guaranteed
Mouse Monoclonal [clone 6E9] (IgG2a) to Human POLR2J2
Human
Western blot, Flow Cytometry
Unconjugated
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2J2 antibody, and then analyzed by flow cytometry.

Details

Human POLR2J2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [6E9]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

POLR2J2 antibody was raised against full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.
Human POLR2J2

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About POLR2J2

Q9GZM3 NM_032959 NP_116581.3

POLR2J2 Antibody

POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes.
(More About POLR2J2)

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2J2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2J2 antibody, and then analyzed by flow cytometry.
Requested From: 
Date Requested: 4/21/2018
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