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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLR2G / RPB7 Antibody (aa1‑122) LS‑C186029
RPB7 antibody LS-C186029 is an unconjugated rabbit polyclonal antibody to RPB7 (POLR2G) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C186029-100
100 µl (1 mg/ml)
Unavailable

Popular POLR2G / RPB7 Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot, Flow Cytometry
Western blot of immunized recombinant protein using POLR2G antibody.
Species: Human, Mouse, Rat
Applications: Western blot, Flow Cytometry, Dot Blot
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Western blot analysis of immunized recombinant protein, using anti-POLR2G monoclonal antibody.
Species: Human, Mouse, Rat
Applications: Western blot, Flow Cytometry, Dot Blot

Product Description

RPB7 antibody LS-C186029 is an unconjugated rabbit polyclonal antibody to RPB7 (POLR2G) from human, mouse, rat and other species. Validated for IHC and WB.
About POLR2G / RPB7
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. P62487 NM_002696 NP_002687.1

POLR2G Antibody, HRPB19 Antibody, RPB19 Antibody, RNA polymerase II subunit B7 Antibody, HsRPB7 Antibody, RPB7 Antibody


Specifications

Target
Human POLR2G / RPB7
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
POLR2G / RPB7 antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 122 (P62487) of RNA polymerase IIG. Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%); Xenopus (98%).
Epitope
aa1-122
Specificity
Human POLR2G / RPB7
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.
IHC of paraffin-embedded CL1-5 xenograft using POLR2G antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: A431. 12% SDS PAGE. POLR2G / RPB7 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/21/2018

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