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Anti-POLL / DNA Polymerase Lambda Antibody (aa563-575) IHC-plus™ LS-B2284

Note: This antibody replaces LS-C55301

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular POLL / DNA Polymerase Lambda Antibodies

Anti-POLL / DNA Polymerase Lambda Antibody (aa563-575) IHC-plus™ LS-B2284
Goat Polyclonal to Human POLL / DNA Polymerase Lambda
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Pig, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLL / DNA Polymerase Lambda Antibody LS-C409943
Rabbit Polyclonal (IgG) to Human POLL / DNA Polymerase Lambda
Human, Mouse
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human POLL / DNA Polymerase Lambda
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Pig, Rabbit
IHC - Paraffin, ELISA
Unconjugated

Details

Human POLL / DNA Polymerase Lambda
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
Dog, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ELISA (1:32000)

Specificity and Use

POLL / DNA Polymerase Lambda antibody was raised against synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%); Dog, Opossum, Turkey, Chicken (92%); Xenopus, Zebrafish, Sea anemone (85%).
LS-E25652 - Lyophilized - 100 µg - $145.00
aa563-575
Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Immunohistochemistry: LS-B2284 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2284 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About POLL / DNA Polymerase Lambda

Q9UGP5 NM_013274 NP_037406.1

POLL Antibody, DNA polymerase beta-2 Antibody, DNA polymerase beta-N Antibody, DNA polymerase lambda Antibody, DNA polymerase kappa Antibody, Pol beta2 Antibody, BETAN Antibody, Pol Lambda Antibody, POLKAPPA Antibody

Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.

Immunohistochemistry

Immunohistochemistry
Anti-POLL antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2284 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/3/2016

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