Target
Human POLL / DNA Polymerase Lambda
Synonyms
POLL | DNA polymerase beta-2 | DNA polymerase beta-N | DNA polymerase lambda | DNA polymerase kappa | Pol beta2 | BETAN | Pol Lambda | POLKAPPA
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-LGLPYREPAERDW, from the C-Terminus of protein sequence according to NP_037406.1.
Specificity
Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Usage
Immunohistochemistry: LS-B2284 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2284 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLL / DNA Polymerase Lambda