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Anti-POLB / DNA Polymerase Beta Antibody (C-Terminus) LS-C153861


Wt. Vol. Conc. Price
100 µg - - $385
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular POLB / DNA Polymerase Beta Antibodies

Anti-POLB / DNA Polymerase Beta Antibody (clone 61, Azide-free) LS-C87746
Mouse Monoclonal [clone 61] (IgG2a) to Human POLB / DNA Polymerase Beta
Western blot
Unconjugated, Azide-free
Anti-POLB / DNA Polymerase Beta Antibody (clone 18S, Azide-free) LS-C88356
Mouse Monoclonal [clone 18S] (IgG1) to Rat POLB / DNA Polymerase Beta
Rat, Human, Mouse, Bovine, Hamster, Xenopus
Western blot, Immunoprecipitation
Unconjugated, Azide-free
Anti-POLB / DNA Polymerase Beta Antibody (aa286-335) IHC-plus™ LS-B7021
Rabbit Polyclonal (IgG) to Human POLB / DNA Polymerase Beta
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal (IgG) to Human POLB / DNA Polymerase Beta
Western blot, ELISA


Human POLB / DNA Polymerase Beta
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • Western blot (0.5 µg/ml)

Specificity and Use

POLB / DNA Polymerase Beta antibody was raised against this affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POL protein.
This antibody reacts with overexpressed and endogenous POLbeta protein. A BLAST analysis was used to suggest reactivity with POLbeta from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLbeta from other sources has not been determined.
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kD in size corresponding to POLbeta by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (2)

Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. Sobol RW, Foley JF, Nyska A, Davidson MG, Wilson SH. DNA repair. 2003 2:609-22. [PubMed:12713817]
DNA polymerase beta-dependent long patch base excision repair in living cells. Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH. DNA repair. 2010 9:109-19. [PubMed:20006562] [PMC:PMC2819632]

About POLB / DNA Polymerase Beta

P06746 NM_002690 NP_002681.1

POLB Antibody, DNA pol beta Antibody, Mutant DNA polymerase beta Antibody, DNA polymerase beta Antibody, DNA polymerase beta subunit Antibody

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

Western blot

Western blot
Western blot of Rabbit Anti-POL (DNA polymerase beta) Antibody (LS-C153861). Lane 1: LN428 FLAG POL (control). Lane 2: A172. Load: 35 ug per lane. Primary antibody: POL antibody at 1:2000 for overnight at 4C. Secondary antibody: IRDye800 goat anti-rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.

Requested From: United States
Date Requested: 10/24/2016

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