Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
PNPT1 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 783 of PNPase (SwissProt Q8TCS8). Percent identity by BLAST analysis: Human (100%); Xenopus (80%).
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs.
IHC of paraffin-embedded Colon ca using PNPase antibody at 1:250 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using PNPase antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2500.
Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. PNPT1 antibody diluted at 1:1000.
Requested From: United States
Date Requested: 2/24/2017