LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PML Antibody (aa1‑581) LS‑C9654
PML antibody LS-C9654 is an unconjugated mouse monoclonal antibody to mouse PML. Validated for ICC and WB.
Antibody
Catalog
Size
Price
LS-C9654-200
200 µg (1 mg/ml)
$605

Popular PML Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-PML antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3501 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Guinea pig, Horse
Applications: IHC, IHC - Paraffin, Western blot
Immunocytochemistry Image
Species: Mouse
Applications: ICC, Western blot
Western blot of recombinant PML.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

PML antibody LS-C9654 is an unconjugated mouse monoclonal antibody to mouse PML. Validated for ICC and WB.
About PML
Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. P29590 NM_002675 NP_002666.1

PML Antibody, PP8675 Antibody, Promyelocytic leukemia Antibody, Protein PML Antibody, TRIM19 Antibody, Promyelocytic leukemia protein Antibody, RING finger protein 71 Antibody, MYL Antibody, RNF71 Antibody


Specifications

Target
Mouse PML
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot (0.5 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PML antibody was raised against 6His fusion protein corresponding to aa1-581 of mouse PML (promyelocytic leukemia protein).
Epitope
aa1-581
Specificity
Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not cross-react with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Usage
Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-1 ug/ml of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 7/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number