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Anti-PLXNA3 / Plexin A3 Antibody (aa34-150) LS-C38284

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $650
Inquire for larger quantities

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Most Popular PLXNA3 / Plexin A3 Antibodies

Anti-PLXNA3 / Plexin A3 Antibody (aa34-150) LS-C38284
Goat Polyclonal (IgG) to Mouse PLXNA3 / Plexin A3
Mouse
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse PLXNA3 / Plexin A3
Mouse
IHC, Western blot, ELISA
Unconjugated

Details

Mouse PLXNA3 / Plexin A3
Goat
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA

Specificity and Use

PLXNA3 / Plexin A3 antibody was raised against e. coli-derived recombinant mouse Plexin A3 (aa34-150).
aa34-150
Selected for its ability to recognize mouse Plexin A3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rmPlexin A1, rmPlexin A2, rhPlexin B1, rhPlexin C1, and rhPlexin D1.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse Plexin A3. The detection limit for rmPlexin A3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody has been used at a concentration of 10 ug/ml to detect Plexin A3 in cryosections of E13 mouse embryos. Tissue was fixed overnight in 4%PFA+14%picric acid in PBS at 4? C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 minutes at room temperature. After blocking, sections were incubated with primary antibody for 3 hours at room temperature followed by NorthernLights 557 donkey anti-goat lgG at room temperature for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Plexin A3. The detection limit for rmPlexin A3 is approximately 1.0 ng/well.

Packaging

Lyophilized.
100 µl deionized water
For research use only.

About PLXNA3 / Plexin A3

P51805 X87852 CAA61132.1

PLXNA3 Antibody, 6.3 Antibody, HSSEXGENE Antibody, Plexin-A3 Antibody, SEX Antibody, Plexin A3 Antibody, Plexin-4 Antibody, PLXN3 Antibody, PLXN4 Antibody, XAP-6 Antibody, Semaphorin receptor SEX Antibody

Coreceptor for SEMA3A and SEMA3F. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Regulates the migration of sympathetic neurons, but not of neural crest precursors. Required for normal dendrite spine morphology in pyramidal neurons. May play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system.

Requested From: United States
Date Requested: 12/4/2016

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