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Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) IHC-plus™ LS-B50

Note: This antibody replaces LS-C18893, LS-C9642


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular PLK1 / PLK-1 Antibodies

Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) IHC-plus™ LS-B50
Rabbit Polyclonal to Human PLK1 / PLK-1
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B4225
Rabbit Polyclonal to Human PLK1 / PLK-1
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-PLK1 / PLK-1 Antibody (aa574-603, Biotin) LS-C266558
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
IHC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human PLK1 / PLK-1
IHC - Paraffin, Western blot, ELISA


Human PLK1 / PLK-1
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:200 - 1:2000)
  • ELISA (1:3000 - 1:12000)

Specificity and Use

Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.
Immunohistochemistry: LS-B50 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B50 was determined to be 10 ug/ml.Western Blot: It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

Mechanism of the interaction of beta(2)-glycoprotein I with negatively charged phospholipid membranes. Hammel M, Schwarzenbacher R, Gries A, Kostner GM, Laggner P, Prassl R. Biochemistry. Nov;40:14173-81. [Full Text Article] [PubMed:11714270]

About PLK1 / PLK-1

P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.


Anti-PLK1 antibody IHC of human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B50 concentration 10 ug/ml.

Western blot

Western blot
Anti-plk-1 Antibody - Western Blot. Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants. Personal communication Hai Jiang, Northwestern Univ.

Western blot

Western blot
Anti-Plk-1 pT210 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kD corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.

Requested From: United States
Date Requested: 10/28/2016

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