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Anti-PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) IHC-plus™ LS-B1675

Note: This antibody replaces LS-C41059
Catalog Size Price
LS-B1675-50 50 µg (0.5 mg/ml) Unavailable

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Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) IHC-plus™ LS-B50
Rabbit Polyclonal to Human PLK1 / PLK-1
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IHC, IHC - Paraffin, Western blot, ELISA
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Anti-PLK1 antibody IHC of human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B50 concentration 10 ug/ml.
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B1674
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
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Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1674 dilution 1:50.
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) IHC-plus™ LS-B1675
Mouse Monoclonal [clone 2A3] (IgG1) to Human PLK1 / PLK-1
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IHC, IHC - Paraffin, Western blot
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Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml.
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B4225
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Anti-PLK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4225 concentration 10 ug/ml.
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B6646
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Anti-PLK1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6646 concentration 5 ug/ml.

100% Guaranteed Publications (1)
Mouse Monoclonal [clone 2A3] (IgG1) to Human PLK1 / PLK-1
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml. Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.
Western blot

Details

Human PLK1 / PLK-1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2A3]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (15 µg/ml)
  • Western blot

Specificity and Use

PLK1 / PLK-1 antibody was raised against synthetic peptide from human PLK1.
pThr210
Modified peptide
Immunohistochemistry: LS-B1675 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1675 was determined to be 15 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced from tissue culture supernatant.
+4°C, avoid freezing.
For research use only.

Publications (1)

1
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Molli PR, Li DQ, Bagheri-Yarmand R, Pakala SB, Katayama H, Sen S, Iyer J, Chernoff J, Tsai MY, Nair SS, Kumar R. The Journal of cell biology. 2010 190:101-14. [PubMed:20603326] [PMC:PMC2911675]

About PLK1 / PLK-1

P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.
(More About PLK1 / PLK-1)

Immunohistochemistry

Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml.
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml.

Western blot

Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.
Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.

Western blot

Western blot
 
Requested From: 
Date Requested: 4/23/2018
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