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Anti-PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) IHC-plus™ LS-B1675
Note: This antibody replaces LS-C41059Most Popular PLK1 / PLK-1 Antibodies
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Details
| Human PLK1 / PLK-1 | |
| Mouse | |
| Human (tested or 100% immunogen sequence identity) | |
| IgG1 Monoclonal [2A3] | |
| Unconjugated | |
| Affinity purified | |
| Unmodified |
Applications
- IHC - Paraffin (15 µg/ml)
- Western blot
Specificity and Use
| PLK1 / PLK-1 antibody was raised against synthetic peptide from human PLK1. | |
| pThr210 | |
| Modified peptide | |
| Immunohistochemistry: LS-B1675 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1675 was determined to be 15 ug/ml. |
Packaging
| Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced from tissue culture supernatant. | |
| +4°C, avoid freezing. | |
| For research use only. |
Publications (1)
1
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Molli PR, Li DQ, Bagheri-Yarmand R, Pakala SB, Katayama H, Sen S, Iyer J, Chernoff J, Tsai MY, Nair SS, Kumar R. The Journal of cell biology. 2010 190:101-14.
[PubMed:20603326]
[PMC:PMC2911675]
About PLK1 / PLK-1
P53350
NM_005030
NP_005021.2
PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.
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Requested From:
Date Requested: 10/18/2017
Date Requested: 10/18/2017
